Xiao-Yan Dong

Dr. Eng., Professor
Department of Biochemical Engineering,
School of Chemical Engineering and Technology,
Tianjin University, Tianjin 300354, P. R. China.

Email: d_xy@tju.edu.cn

B.Sc. –1982, Department of Biochemistry, Qinghai Institute of Veterinary Medicine, Xining, China
M.Sc. – 1990, Graduate School of Agriculture, Shinshu University, Nagano, Japan
Dr. Eng. – 2005, Department of Biochem. Eng., Tianjin University, Tianjin, China
Research Interests

Kinetic structures of amyloid proteins
Design of Inhibitors against amyloid protein aggregation
Protein refolding and purification
Genetic engineering

Professional Activities

1982 – 1986 Research Associate, Qinghai Academy of Veterinary Medicine
1986 – 1987 Research Fellow, Tokyo Institute of Agriculture and Technology
1990 – 1991 Research Fellow, High Separation Center, Soken Chem Eng Co, Tokyo
1991 – 1996 Lecturer, Department of Biochemical Engineering, TJU
1995 – 1996 Visiting Scientist, Faculty of Agriculture, Kyushu University, Japan
1996 – 2002 Associate Professor, Department of Biochemical Engineering, TJU
2002 – present Professor, Department of Biochemical Engineering, TJU

Selected Publications
  1. X.-Y. Dong, X.-D. Feng, Y. Sun*: His-tagged protein purification by metal-chelate affinity extraction with nickel-chelate reverse micelles. Biotechnology Progress, 2010, 26 (4): 1088-1094.
  2. X.-Y. Dong, Y. Meng, X.-D. Feng, Y. Sun*: A metal-chelate affinity reverse micellar system for protein extraction. Biotechnology Progress, 2010, 26 (1): 150-158.
  3. X.-Y. Dong, L.-J. Chen, Y. Sun*: Effect of operating conditions on the refolding of his-tagged enhanced green fluorescent protein by artificial chaperone-assisted metal affinity chromatography. Biochemical Engineering Journal, 2009, 48 (1): 65-70.
  4. X.-Y. Dong, J.-H. Liu, F.-F. Liu, Y. Sun*: Self-interaction of native and denatured lysozyme in the presence of osmolytes, l-arginine and guanidine hydrochloride.Biochemical Engineering Journal, 2009, 43 (3): 321-326.
  5. X.-Y. Dong, L.-J. Chen, Y. Sun*: Refolding and purification of histidine-tagged protein by artificial chaperone-assisted metal affinity chromatography. Journal of Chromatography A, 2009, 1216 (27): 5207-5213.
  6. X.-Y. Dong, Fu M.-L., Y. Sun*: Refolding of recombinant homodimeric malate dehydrogenase expressed in Escherichia coli as inclusion bodies. Biochemical Engineering Journal, 2008, 38 (3): 341-348.
  7. Y. Liu, X.-Y. Dong, Y. Sun*: Protein separation by affinity extraction with reversed micelles of Span 85 modified with Cibacron Blue F3G-A. Separation and Purification Technology, 2007, 53 (2) 289–295.
  8. X.-Y. Wu, Y. Liu, X.-Y. Dong, Y. Sun*: Protein refolding mediated by reversed micelles of Cibacron Blue F-3GA modified nonionic surfactant. Biotechnology Progress, 2006, 22 (2): 499-504.
  9. X.-Y Dong, Wu, Y. Sun*: Refolding of denatured lysozyme assisted by artificial chaperones in reverse micelles. Biochemical Engineering Journal, 2006, 31 (1): 92-95.
  10. H.-Q. Yu, X.-Y. Dong, Y. Sun*: An alternating elution strategy for screening high affinity peptides from a phage display peptide library. Biochemical Engineering Journal, 2004, 18 (3): 169-175.
  11. X.-Y. Dong, G.Q. Shi, W. Li, Y. Sun*: Modeling and simulation of fed-batch protein refolding process. Biotechnology Progress, 2004, 20 (4): 1213-1219.
  12. X.-Y. Dong, Y. Huang, Y. Sun*: Refolding kinetics of denatured-reduced lysozyme in the presence of folding aids. Journal of Biotechnology, 2004, 114 (1-2): 135-142.