Xiao-Yan Dong

Dr. Eng., Professor
Department of Biochemical Engineering,
School of Chemical Engineering and Technology,
Tianjin University, Tianjin 300072, P. R. China.

Email: d_xy@tju.edu.cn

B.Sc. –1982, Department of Biochemistry, Qinghai Institute of Veterinary Medicine, Xining, China
M.Sc. – 1990, Graduate School of Agriculture, Shinshu University, Nagano, Japan
Dr. Eng. – 2005, Department of Biochem. Eng., Tianjin University, Tianjin, China
Research Interests

Protein refolding and purification
Protein stabilization
Genetic engineering

Professional Activities

1982 – 1986 Research Associate, Qinghai Academy of Veterinary Medicine
1986 – 1987 Research Fellow, Tokyo Institute of Agriculture and Technology
1990 – 1991 Research Fellow, High Separation Center, Soken Chem Eng Co, Tokyo
1991 – 1996 Lecturer, Department of Biochemical Engineering, TJU
1995 – 1996 Visiting Scientist, Faculty of Agriculture, Kyushu University, Japan
1996 – 2002 Associate Professor, Department of Biochemical Engineering, TJU
2002 – present Professor, Department of Biochemical Engineering, TJU

Selected Publications
  1. X.-Y. Dong, X.-D. Feng, Y. Sun*: His-tagged protein purification by metal-chelate affinity extraction with nickel-chelate reverse micelles. Biotechnology Progress, 2010, 26 (4): 1088-1094.
  2. X.-Y. Dong, Y. Meng, X.-D. Feng, Y. Sun*: A metal-chelate affinity reverse micellar system for protein extraction. Biotechnology Progress, 2010, 26 (1): 150-158.
  3. X.-Y. Dong, L.-J. Chen, Y. Sun*: Effect of operating conditions on the refolding of his-tagged enhanced green fluorescent protein by artificial chaperone-assisted metal affinity chromatography. Biochemical Engineering Journal, 2009, 48 (1): 65-70.
  4. X.-Y. Dong, J.-H. Liu, F.-F. Liu, Y. Sun*: Self-interaction of native and denatured lysozyme in the presence of osmolytes, l-arginine and guanidine hydrochloride.Biochemical Engineering Journal, 2009, 43 (3): 321-326.
  5. X.-Y. Dong, L.-J. Chen, Y. Sun*: Refolding and purification of histidine-tagged protein by artificial chaperone-assisted metal affinity chromatography. Journal of Chromatography A, 2009, 1216 (27): 5207-5213.
  6. X.-Y. Dong, Fu M.-L., Y. Sun*: Refolding of recombinant homodimeric malate dehydrogenase expressed in Escherichia coli as inclusion bodies. Biochemical Engineering Journal, 2008, 38 (3): 341-348.
  7. Y. Liu, X.-Y. Dong, Y. Sun*: Protein separation by affinity extraction with reversed micelles of Span 85 modified with Cibacron Blue F3G-A. Separation and Purification Technology, 2007, 53 (2) 289–295.
  8. X.-Y. Wu, Y. Liu, X.-Y. Dong, Y. Sun*: Protein refolding mediated by reversed micelles of Cibacron Blue F-3GA modified nonionic surfactant. Biotechnology Progress, 2006, 22 (2): 499-504.
  9. X.-Y Dong, Wu, Y. Sun*: Refolding of denatured lysozyme assisted by artificial chaperones in reverse micelles. Biochemical Engineering Journal, 2006, 31 (1): 92-95.
  10. H.-Q. Yu, X.-Y. Dong, Y. Sun*: An alternating elution strategy for screening high affinity peptides from a phage display peptide library. Biochemical Engineering Journal, 2004, 18 (3): 169-175.
  11. X.-Y. Dong, G.Q. Shi, W. Li, Y. Sun*: Modeling and simulation of fed-batch protein refolding process. Biotechnology Progress, 2004, 20 (4): 1213-1219.
  12. X.-Y. Dong, Y. Huang, Y. Sun*: Refolding kinetics of denatured-reduced lysozyme in the presence of folding aids. Journal of Biotechnology, 2004, 114 (1-2): 135-142.